Alcohol Dehydrogenase Antibody Properties Description Anti-ALCOHOL DEHYDROGENASE (Yeast) (RABBIT) Antibody - 100-4143 Host Rabbit Target Species Yeast Known Cross Reactivity Saccharomyces cerevisiae (Bakers yeast) Clonality Polyclonal Application ELISA : 1:5,000 - 1:25,000 Western Blot : 1:500 - 1:2,000 ImmunoPrecipitation: 1:100 Other Dilution: User Optimized Physical State Lyophilized Shipping Condition Ambient Format Antiserum Label Unconjugated Concentration 90 mg/mL by Refractometry Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Reconstitution Volume 2.0 mL Reconstitution Buffer Restore with deionized water (or equivalent) Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName ADH1, ADC1, YOL086C Relevant Links UniProtKB - P00330NCBI - 12643994GeneID - 2538902

Alcohol Dehydrogenase Antibody Description

Background Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone.  Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer.  This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization.  Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287. Synonyms rabbit anti-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1 Immunogen This antibody was prepared from whole rabbit serum produced by repeated immunizations with full length Alcohol Dehydrogenase isolated from yeast. Immunogen Type Native Protein Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Application Note This antibody has been tested for use in ELISA and by western blot. Purity/Specifity This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alcohol Dehydrogenase [Yeast].  Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis.   Cross reactivity with Alcohol Dehydrogenase from other sources is unknown. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.