Alanine Transaminase Antibody Biotin Conjugated Properties Description Anti-ALANINE TRANSAMINASE (ALT) (SHEEP) Antibody Biotin Conjugated- 200-606-206S Host Sheep Target Species Swine Known Cross Reactivity swine Clonality Polyclonal Application ELISA : 1:5,000 - 1:25,000 Western Blot : 1:500 - 1:3,000 Other Dilution: User Optimized Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Biotin Concentration 1.0 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative 0.01% (w/v) Sodium Azide Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free GeneName ALT Relevant Links UniProtKB - P13191NCBI - 113565GeneID - 2875

Alanine Transaminase Antibody Biotin Conjugated Description

Background Anti-Alanine Transaminase antibody is part of the class-I pyridoxal-phosphate-dependent aminotransferase family. It catalyzes the transfer of the alanine amino group, reversible transamination, to produce pyruvate and glutamate. Located in the cytoplasm, this antibody is a factor in cellular nitrogen metabolism and liver gluconeogenesis. Anti-Alanine Transaminase antibody is ideal for investigators interested in Metabolism, Signal Transduction, and Cancer research. Synonyms sheep anti-Alanine Transaminase Antibody, biotin conjugated sheep anti-Alanine Transaminase Antibody, AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody Immunogen Alanine Transaminase (ALT) [Pig Heart] Immunogen Type Native Protein Storage Condition Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Application Note Anti-Alanine Transaminase has been assayed against 1.0 ug of Alanine Transaminase (ALT) in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Alanine Transaminase (ALT) in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Purity/Specifity Alanine Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Alanine Transaminase (ALT) [Pig Heart].  Cross reactivity against Alanine Transaminase (ALT) from other sources is unknown. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.