Rockland/AKT2 Antibody/600-401-425/100 µg,Rockland,,Rockland,rockland-inc,Rockland Immunochemicals Inc,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Rockland/AKT2 Antibody/600-401-425/100 µg

产品编号：600-401-425

市场价：¥7180.00

场地：美国(厂家直采)

产品分类：抗体类>一抗>功能性抗体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$359.00

品牌： Rockland

公司：Rockland Immunochemicals Inc.

公司分类：

商品介绍

AKT2AntibodyProperties

Description

Anti-AKT2(RABBIT)Antibody-600-401-425

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Polyclonal

Application

ELISA:1:4000-1:16000
FlowCytometry:UserOptimized
WesternBlot:1:1000
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Unconjugated

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

StABIlizer

None

GeneName

AKT2

RelevantLinks

UniProtKB-P31751
GeneID-208
GeneID-164731

AKT2AntibodyDescription

Background

AKT2isalsoknownasV-AKTMurineThymomaViralOncogeneHomolog2antibody,Racproteinkinasebetaantibody,PKBbetaantibodyorPRKBBantibody. AKT2isanisoformofthephosphoinositide-dependentserine-threonineproteinkinaseAKTandisenrichedininsulin-responsivetissuesandhasbeenimplicatedinthemetabolicactionsofthehormone. AKT2isaputativeoncogeneencodingaproteinbelongingtoasubfamilyofserine/threoninekinasescontainingSH2-like(Srchomology2-like)domains.FurThermore,AKT2wasshowntobeamplifiedandoverexpressedin2of8ovariancarcinomacelllinesand2of15primaryovariantumors.Over-expressionofAKT2contributestothemalignantphenotypeofasubsetofhumanductalpancreaticcancers.AKT2isageneralproteinkinasecapableofphophorylatingseveralknownproteins. AKT2mediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin). PI3-kinaserecruitsAKT2tothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKT2dissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus. AKT2hastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.

Synonyms

rabbitanti-AKT2antibody,AKT2,AKT-2,PKBantibody,PKBbetaantibody,PKBBETAantibody,PRKBBantibody,ProteinkinaseAkt2antibody,ProteinkinaseBbetaantibody,RAC-betaserine/threonine-proteinkinase,RAC-PK-beta

Immunogen

Thisaffinitypurifiedantibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaa455-468(of481)ofhumanAKT2conjugatedtoKeyholeLimpetHemocyanin(KLH).

ImmunogenType

Peptide

StorageCondition

Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

ThisantibodywastestedbyELISAandimmunoblottingandwasfoundtobereactivewithbothunphosphorylatedandphosphorylatedAKT2inalysateofHEK293cells. Althoughnottested,thisantibodyislikelyfunctionalinimmunohistochemistryandimmunoprecipitation.ThisproducthasbeenassayedbyimmunoblotagainstaHEK293celllysateandisreactiveata1:1,000dilutionshowingabandatapproximately60kDa. Aworkingdilutionof1:4,000to1:16,000issuggestedforthisproductinastandardcaptureELISAusingTMB(3,3",5,5"-Tetramethylbenizidine)code#TMBE-100asasubstratefor30minutesatroomtemperatureagainst0.1ugoftheimmunizingpeptide.Researchersshoulddetermineoptimaltitersforotherapplications.

Purity/Specifity

ThisaffinitypurifiedantibodyisdirectedagainsthumanAKT2.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Theproductwasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitypurification. CrossreactivitywithAKT2fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows85%identitytomouseand92%identitywithrat,therefore,crossreactivityisexpected.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

品牌介绍

ROCKLAND

Rockland Immunochemicals Inc. 成立于1962年，是一家全球性的生物技术公司，为功能基因组学，基因治疗和药物发现市场的基础，应用和临床研究制造领先的研究工具。

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