Rockland/Akt phospho S473 Antibody/200-301-268/100 µg,Rockland,,Rockland,rockland-inc,Rockland Immunochemicals Inc,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Rockland/Akt phospho S473 Antibody/200-301-268/100 µg

产品编号：200-301-268

市场价：¥7180.00

场地：美国(厂家直采)

产品分类：抗体类>一抗>功能性抗体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$359.00

品牌： Rockland

公司：Rockland Immunochemicals Inc.

公司分类：

商品介绍

AktphosphoS473AntibodyProperties

Description

Anti-AKTpS473(MOUSE)MonoclonalAntibody-200-301-268

Host

Mouse

TargetSpecies

Human

KnownCrossReactivity

human,mouse,rat,monkey

Clonality

Monoclonal17F6.B11IgG1kappa

Application

ELISA:1:20,000
IFMicroscopy:1:500-1:3,000
FlowCytometry:UserOptimized
WesternBlot:1:500-1:3,000
Immunohistochemistry:20µg/mL
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG1

Label

Unconjugated

Concentration

1.0mg/mlbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

StABIlizer

None

GeneName

AKT1

RelevantLinks

UniProtKB-P31749
NCBI-62241011
GeneID-207

AktphosphoS473AntibodyDescription

Background

PhosphoAKTantibodyissuitableforELISA,immunohistochemistry,immunoprecipitationandwesternblotting.AKTphospho473isacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKTpS473(MOUSE)MonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.

Synonyms

mouseanti-AKTpS473Antibody,phosphoAKT,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1

Immunogen

Anti-AKTpS473(MOUSE)MonoclonalAntibodywasproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoresiduessurroundingS473ofhumanAKT1protein,followedbyhybridomadevelopment.

ImmunogenType

Peptide

PostTranslationalModification

Phosphorylation

StorageCondition

StoreAnti-AKTpS473(MOUSE)MonoclonalAntibodyat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

Expectabandapproximately56kDainsizecorrespondingtophosphorylatedAKTproteinbywesternblottingintheappropriatecelllysateorextract.Thisphospho-specificmonoclonalantibodyreactswithhumanandmouseAKTpS473andshowsminimalreactivitybyELISAagainstthenon-phosphorylatedformoftheimmunizingpeptide.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistryuseformalin-fixedparaffin-embeddedsections.Nopre-treatmentofsampleisrequired.

Purity/Specifity

Anti-AKTpS473MonoclonalAntibodywaspurifiedfromconcentratedtissueculturesupernatebyProteinAchromatography.ThisphosphospecificmonoclonalantibodyisspecificforphosphorylatedhumanandmouseAKTproteinatS473.ABLASTanalysiswasusedtosuggestcross-reactivitywithAKTpS473fromhuman,mouse,ratandchimpanzeesourcesbasedon100%homologywiththeimmunizingsequence.Cross-reactivitywithAKTfromothersourceshasnotbeendetermined.Cross-reactivitywithAKT2andAKT3hasnotbeendetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

品牌介绍

ROCKLAND

Rockland Immunochemicals Inc. 成立于1962年，是一家全球性的生物技术公司，为功能基因组学，基因治疗和药物发现市场的基础，应用和临床研究制造领先的研究工具。

产品列表：

No.	品牌	货号	名称	规格
1	ROCKLAND	100-4191	Superoxide Dismutase Antibody	2mL
2	ROCKLAND	100-4140	Acid Phosphatase Antibody	2ml
3	ROCKLAND	200-301-U98	5-mC Antibody	50ug
4	ROCKLAND	200-301-065s	NF-kB p65 Antibody	25ul
5	ROCKLAND	200-301-065	NF-kB p65 Antibody	100ug
6	ROCKLAND	200-303-400	ATM phospho S1981 Peroxidase Conjugated Antibody	100ug
7	ROCKLAND	600-401-952	IRAK-2 Antibody	100ug
8	ROCKLAND	609-1606	Human IgA (alpha chain) Antibody Biotin Conjugated	1mg
9	ROCKLAND	600-401-147	Alpha-1-Trypsin Antibody	1mg
10	ROCKLAND	610-4610	Mouse κ (kappa chain) Antibody Biotin Conjugated	1mg
11	ROCKLAND	609-1316	Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed	2mg
12	ROCKLAND	613-4528	Sheep IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed	500ug
13	ROCKLAND	805-7602	Fab Goat IgG Antibody Biotin Conjugated	1mg
14	ROCKLAND	714-406-002	F(ab')2 Swine IgG (H&L) Antibody Biotin Conjugated	500ug
15	ROCKLAND	600-401-147S	Alpha-1-Trypsin Antibody	25ul