Rockland/AKT3 Antibody/200-301-J36S/25 µL,Rockland,,Rockland,rockland-inc,Rockland Immunochemicals Inc,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Rockland/AKT3 Antibody/200-301-J36S/25 µL

产品编号：200-301-J36S

市场价：¥1980.00

场地：美国(厂家直采)

产品分类：抗体类>一抗>功能性抗体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$99.00

品牌： Rockland

公司：Rockland Immunochemicals Inc.

公司分类：

商品介绍

AKT3AntibodyProperties

Description

Anti-AKT3(MOUSE)MonoclonalAntibody-200-301-J36S

Host

Mouse

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Monoclonal9A5.H9.G7IgG1kappa

Application

ELISA:1:2,000-1:10,000
FlowCytometry:UserOptimized
WesternBlot:1:500-1:2000
Immunohistochemistry:20µg/mL
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG1

Label

Unconjugated

Concentration

1mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

StABIlizer

None

GeneName

AKT3

RelevantLinks

GeneID-10000
TheAktsignalingpathway:Anemergingtherapeutictargetinmalignantmelanoma.MadhunapantulaSV,etal.CancerBiolTher.2011Dec15;12(12)
TheAktkinases:isoformspecificityinmetabolismandcancer.GonzalezE,McGrawTE.CellCycle.2009Aug15;8(16):2502-8
ThePTEN-AKT3signalingcascadeasatherapeutictargetinmelanoma.MadhunapantulaSV,RobertsonGP.PigmentCellMelanomaRes.2009Aug;22(4):400-19.
UniProtKB-Q9Y243
NCBI-Q9Y243

AKT3AntibodyDescription

Background

AKT3AntibodydetectsAKT3whichisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT3AntibodyisidealforinvestigatorsinvolvedinCellSignaling,NeuroscienceandSignalTransductionresearch.

Synonyms

Mouseanti-AKT3antibody,AKT-3,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt3antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma

Immunogen

Anti-AKT3AntibodywasproducedinmicebyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT3protein.

ImmunogenType

Peptide

StorageCondition

Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.

ApplicationNote

Anti-AKT3AntibodyissuitableforELISA,immunohistochemistry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT3proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.

Purity/Specifity

Anti-AKT3antibodyisdirectedagainsthumanAKT3.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT3antibodywaspurifiedfromascitesbyProteinAchromatography.CrossreactivitywithAKT3fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows100%identitytohuman,mouse,andrat,therefore,crossreactivityisexpected.Cross-reactivitywithAKT2andAKThasnotbeendetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

品牌介绍

ROCKLAND

Rockland Immunochemicals Inc. 成立于1962年，是一家全球性的生物技术公司，为功能基因组学，基因治疗和药物发现市场的基础，应用和临床研究制造领先的研究工具。

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