Rockland/NFkB p65 (RelA) Phospho S276 Antibody/100-401-264/100 µL,Rockland,,Rockland,rockland-inc,Rockland Immunochemicals Inc,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Rockland/NFkB p65 (RelA) Phospho S276 Antibody/100-401-264/100 µL

产品编号：100-401-264

市场价：¥7180.00

场地：美国(厂家直采)

产品分类：抗体类>一抗>功能性抗体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$359.00

品牌： Rockland

公司：Rockland Immunochemicals Inc.

公司分类：

商品介绍

NFkBp65(RelA)PhosphoS276AntibodyProperties

Description

Anti-NFKBp65(RelA)pS276(RABBIT)Antibody-100-401-264

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Polyclonal

Application

ELISA:1:10,000-1:30,000
WesternBlot:1:1,000-1:5,000
Immunohistochemistry:1:200-1:800
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

Antiserum

Label

Unconjugated

Concentration

80mg/mLbyRefractometry

Buffer

None

Preservative

0.01%(w/v)SodiumAzide

StABIlizer

None

GeneName

RELA

RelevantLinks

UniProtKB-Q04206
NCBI-223468676
GeneID-5970

NFkBp65(RelA)PhosphoS276AntibodyDescription

Background

NF-kBwasoriginallyidentifiedasafactorthatbindstotheimmunoglobulinkappalightchainenhancerinBcells.Itwassubsequentlyfoundinnon-BcellsinaninactivecytoplasmicformconsistingofNF-kBboundtoIkB.NF-kBwasoriginallyidentifiedasaheterodimericDNAbindingproteincomplexconsistingofp65(RelA)andp50(NFKB1)subunits.Otheridentifiedsubunitsincludep52(NFKB2),cRel,andRelB.Thep65,cRel,andRelBsubunitsareresponsIBLefortransactivation.Thep50andp52subunitspossessDNAbindingactivitybutlimitedabilitytotransactivate.p52hasbeenreportedtoformtranscriptionallyactiveheterodimerswiththeNF-kBsubunitp65,similartop50/p65heterodimers.Lowlevelsofp52andp50homodimerscanalsoexistincells.Theheterodimersofp52/p65andp50/p65areregulatedbyphysicalinactivationinthecytoplasmbyIkB-alpha.IkB-alphabindstothep65subunitpreventingnuclearlocalizationandDNAbinding.ActivatorsmediatearapidphosphorylationofIkBbyIkBkinase(IKK)whichresultsinsubsequentubiquitinationandproteolyticdegradation.NF-kBisthentransportedtothenucleus,whereitactivatestranscriptionoftargetgenesthroughbindingtoNF-kBtargetsequenceswithinthepromoter.TheHTLV-IproteinTaxcaninduceconstitutiveNF-kBactivationthroughphosphorylationofbothIkB-alphaandIkB-beta.ThetransformingproteinTaxinhibitsp53transcriptionalactivitythroughtheNFkBsignalingpathway,specificallyviathep65(RelA)subunit.Theinhibitionofp53activityisdependentuponphosphorylationofp65(RelA)atS536bytheupstreamkinaseIKKß.Anti-NFkBantibodyisidealforCellBIOLOGy,NuclearSignaling,NeuroscienceandSignalTransductionResearch.

Synonyms

rabbitanti-NFkBp65pS276Antibody,rabbitanti-RelApS276Antibody,NFKB,nfkb,NF-kB,NF-kappaB,NFkappaB,Anti-NF-kBantibody

Immunogen

NFkBp65(RelA)peptidecorrespondingtoaninternalregionnearphosphoSerine276ofthehumanproteinconjugatedtoKeyholeLimpetHemocyanin(KLH).

ImmunogenType

Peptide

PostTranslationalModification

Phosphorylation

StorageCondition

Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

PhosphoNFkBantibodyreactshumanpS276p65andshowsminimalreactivitybywesternblotwithnon-phosphorylatedp65andminimalreactivitybyELISAagainstthenon-phosphorylatedformoftheimmunizingpeptide. A1:500dilutionhasbeenusedforstainingp65inhumankidneytissuebyIHC.Tissuewasformalinfixedandparaffinembedded.Althoughnottested,thisantibodyislikelyfunctionalinimmunohistochemistryandimmunoprecipitation.Allconditionsmustbeuseroptimized.

Purity/Specifity

Anti-NFkBp65(RelA)PhosphoS276Antibodywaspreparedfrommonospecificantiserumbydelipidationanddefibrination.ThisphosphospecificpolyclonalantibodyisspecificforphosphorylatedpS276humanp65. Reactivitywithnon-phosphorylatedp65isminimal.CrossreactivitywithpS276phosphorylatedp65frommouse,ratorotherspecieshasnotbeendetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

品牌介绍

ROCKLAND

Rockland Immunochemicals Inc. 成立于1962年，是一家全球性的生物技术公司，为功能基因组学，基因治疗和药物发现市场的基础，应用和临床研究制造领先的研究工具。

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